Bowman layer transplantation (BLT) has a good response in patients with advanced progressive keratoconus, improving corneal topography and visual acuity, and stabilizing dilation. BLT is challenging, but in recent years, several studies have elaborated on the efficacy, safety, and stability of BLT, and a technique has recently been developed to locate BL layer grafts on the anatomical BL layer or anterior matrix of the recipient to achieve results comparable to that of BL layer intrastromal grafts, which significantly simplifies this surgical technique and is conducive to the application and promotion of BLT.
Summary:Bowman layer transplantation (BLT) has a good response in patients with advanced progressive keratoconus, improving corneal topography and visual acuity, and stabilizing dilation. BLT is challenging, but in recent years, several studies have elaborated on the efficacy, safety, and stability of BLT, and a technique has recently been developed to locate BL layer grafts on the anatomical BL layer or anterior matrix of the recipient to achieve results comparable to that of BL layer intrastromal grafts, which significantly simplifies this surgical technique and is conducive to the application and promotion of BLT.
Objective:To investigate the prevalence of demodex infectionin dry eye outpatients and analyze its associated influencing factors, aiming to provide guidance for the prevention and management ofdemodex-associated Ocular diseases. Methods: A convenient sampling method was employed to study 229 patients treated in the dry eye clinic of public specialized hospital in Nanchang City, Jiangxi Province from May to November 2023. Questionnaire surveys and microscopic examinations of demodex in eyelashes were conducted. Results: The overall prevalence rate of demodex infection was 47.2%. One-Way ANOVAshowed that Residential environment and frequency of pillowcase and quilt cover changing and facial skin conditions were significantly associated with the presenceof demodex infection.Multiple linear regression analysis revealed that residentialenvironment and facial skin conditions were independent risk factors for developing a higher degree of demodex infection. Residential environment and facial skin conditions together accounted for 19.3% variation in the severity level of demodex infections.The regression equation between independent variablesand dependent variable is:1.644+0.273*residential environment-0.076*facial skin condition. Conclusions: Demodex infection is very common in dry eye outpatients of all ages and both sexes.Ocular demodex infection is more common in people who live in damp, dark environment and have facial skin diseases.
图1为Marfan氏综合征之晶体半脱位的局部特写。散瞳状态下，裂隙灯斜照显示晶状体曲率增加，尤其是后曲率增加更加明显；又加之下方可见丝丝缕缕的悬韧带，细细观察竟能区分出双股分离(晶状体前后两组悬韧带的部分松弛和断裂)，整体望去犹如火焰助推下准备腾空的一枚热气球。此照片的价值在于，能在直视下观察到活体晶状体的前后两组悬韧带，兼具有想象空间的美感。
Blindness prevention has been an important national policy in China. Previous strategies, such as deploying experienced cataract surgeons to rural areas and assisting in building local ophthalmology centers, had successfully decreased the prevalence of visual impairment and blindness. However, new challenges arise with the ageing population and the shift of the disease spectrum towards age-related eye diseases and myopia. With the constant technological boom, digital healthcare innovations in ophthalmology could immensely enhance screening and diagnosing capabilities. Artificial intelligence (AI) and telemedicine have been proven valuable in clinical ophthalmology settings. Moreover, the integration of cutting-edge communication technology and AI in mobile clinics and remote surgeries is on the horizon, potentially revolutionizing blindness prevention and ophthalmic healthcare. The future of blindness prevention in China is poised to undergo significant transformation, driven by emerging challenges and new opportunities.
Objective To explore the use of indocyanine green lens anterior capsular membrane staining in cataract?phacoemulsification and to assess its safety and efficacy. Methods Sixty-six cases (80 eyes) of white cataract patients who underwent cataract phacoemulsification as inpatients in Jiangyin People's Hospital from December 2022 to December 2023 were selected and divided into 40 eyes in the control group and 40 eyes in the stained group according to whether or not the anterior capsule membrane of indocyanine green lens was stained or not in the intraoperative period, and intraoperative recordings were made on the success of capsule tearing, time of capsule tearing, and the occurrence of complications in the two groups, and postoperative follow-ups were made to track the best-corrected visual acuity, intraocular pressure(IOP), corneal endothelial cell counts, and complications in the two groups at the first day, week, month, and month, respectively; and the above indexes were analyzed statistically. Results The intraoperative time for continuous circular capsulorhexis(CCC) in the stained group was significantly less than that in the control group and the success rate of capsular tearing (85%) was significantly higher than that in the control group, both of which were statistically significant (t=6.508, 2=11.168, P<0.05); The incidence of intraoperative radial tearing of the tearing capsule incision, failure to CCC and change the truncated capsule approach, and intraocular lens ciliary sulcus implantation was significantly lower in the stained group than in the control group (2=5.000, 5.230, 4.501, P<0.05); The incidence of postoperative corneal oedema, anterior chamber exudation and elevated IOP in the staining group were not significantly higher in the staining group compared with the control group; there was no significant difference between the best-corrected visual acuity and IOP of the patients of the two groups in the different periods of time. In the staining group, the number of corneal endothelial cells lost in the first week after surgery was less than that in the control group (t=2.708, P<0.05), while the difference in the number of corneal endothelial cells lost in the first month and the third month after surgery was not statistically significant between the two groups. Conclusion Indocyanine green lens anterior capsular membrane staining can improve the success rate of CCC and reduce intraoperative complications in cataract echo-emulsification in the absence of fundus red light reflex without obvious toxic side effects, making it a safe and efficient adjunct.
Abstract Objective To investigate the correlation between the expression level, survival prognosis and immune invasion of TBX2 in uveal melanoma. Methods The expression and clinical features of TBX2 were analyzed by TIMER2.0 database, and the survival and prognosis of TBX2 were analyzed by UCSC database. Then cBioPortal database was used to analyze the mutation survival changes of human TBX2, and BloodSpot and TIMER2.0 databases were used to explore the correlation between TBX2 and cancer immune infiltration. The relationship between its expression and molecular mechanism was studied by single cell State Mapping and Gene Set Variation analysis (GSVA). Results TBX2 mRNA expression levels were significantly changed in 15 tumor types, and TBX2 was a typical prognostic marker for ACC, UVM and KIRP. The mutation had no significant correlation with survival status, but was associated with T cells. In addition, the TBX2 pathway is enriched to ABC transporters, DNA repair, and damage in UVM. Conclusion TBX2 plays a key role in the survival and immune invasion of Uveal melanoma.
Objective: To investigate a technique for fixation and preparation of frozen sections of whole mouse eyeballs. Methods: Three male C57BL/6 mice, aged 2 and 24 months, were taken from the general class and the eyeballs were removed after anaesthesia, killed and fixed in picric acid fixative for 2 hours to prepare frozen sections. Subsequently, hematoxylin-eosin staining was performed and the preparation of frozen sections of whole mouse eyeballs was observed under a light microscope. Results: The mouse eyeballs obtained by this method were basically undistorted, and the whole layer of mouse eyeball sections was observed to be complete and clear under the microscope. The structure of the cornea was clear, the outline of the lens was basically complete, and the ciliary processes and ciliary zonules were visible. The structure of the four cell layers in the retina was clear and without fissures, the nuclei and nuclear membranes were clear and blue, and the cytoplasm of the cells was pink with bright colours. There were many pigment cells in the choroid and the structure was continuous and complete. Conclusions: In conclusion, the frozen sections of mouse eyeballs prepared by the above method were satisfactory and simple, which laid a certain foundation for good scientific research conditions.
The training of professional master students in ophthalmology (professional master) is faced with many restriction. The 3D Surgery Joint Teaching Laboratory teaching method (Wet-Lab) realizes the anatomy of the eye, the rehearsal of the operation and the simulation of the clinical process, etc. Its repeatability and 3D authenticity can not only deepen the learning impression and improve the operation level of the master of ophthalmology, but also greatly increase the learning interest, providing a new teaching mode for training qualified ophthalmologists.
A 30-year-old male patient had poor vision from an early age and planned to undergo transparent lens replacement surgery locally. After eye examination and evaluation, he was diagnosed as bilateral pupil residual membrane and bilateral ametropia. The patient underwent one-stage resection of residual pupil membrane and two-stage TICL implantation, and the visual acuity recovered well after operation. In this paper, the treatment process of this patient was reviewed in order to provide reference for the diagnosis and treatment of clinical ametropia with pupillary residual membrane.
