Objective: To screen the eyes of term neonates and analyze the occurrence and characteristics of eye diseases. Methods: The eye screening results of 4 594 term neonates admitted to the Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University from January 2021 to June 2022 were recorded and retrospectively analyzed. Results: The total abnormal rate of neonatal eye screening was about 10.54%, among which the abnormal rate of fundus was about 6.38%. Common diseases of the anterior segment included neonatal dacryocystitis, conjunctivitis, hemangioma, entropion, cataract, et al. Common diseases of the posterior segment included retinal leukoplakia, retinal hemorrhage, familial exudative retinopathy, et al. Conclusions: The wide-angle infant fundus screening instrument could detect neonatal occult ophthalmopathy early. And some blinding ophthalmopathies might need early treatment. In the future, it is necessary to establish and improve the screeningand the follow-up mechanism system for the neonatal ophthalmopathy.