目的： 探讨EZ Prep清洗液替代二甲苯进行手工脱蜡制作特殊染色片的效果。 方法: 应用EZ Prep清洗液替代二甲苯脱蜡，选取中山大学中山眼科中心临床病理科石蜡标本100例进行回顾性研究，常规切片后切片一式两份，分别采用传统二甲苯和EZ Prep清洗液手工脱蜡后按实验室标准化操作流程进行特殊染色，并比较脱蜡时间和染色效果。 结果： EZ Prep清洗液手工脱蜡处理的切片在革兰氏染色(Gram's)、六胺银染色(GMS)、高碘酸-无色品红染色(PAS)、马松(Masson)三色染色、刚果红等多种特殊染色中的染色质量和二甲苯脱蜡处理的效果一致，两组间优良率比较差异无统计学意义(χ ² = 0. 33，P ＞ 0. 05)，且平均脱蜡时间由(33.0±2.7)min缩短至(7.2±1.1)min，加快了染色出片时间。结论：EZ Prep清洗液可以替代二甲苯在眼组织特殊染色中脱蜡，并具有脱蜡时间短、环保的优点，值得推广应用。

Objective: To investigate the effect of EZ Prep cleaning solution as an alternative to xylene for manual deparaffinization in the preparation of special staining slides. Methods: EZ Prep cleaning solution was utilized to replace xylene for deparaffinization in a retrospective study involving 100 paraffin-embedded specimens from the Clinical Pathology Department of Zhongshan Ophthalmic Center, Sun Yat-sen University. Routine sections were prepared and duplicated, with one set deparaffinized using traditional xylene and the other using EZ Prep cleaning solution. Subsequent special staining was performed following standardized laboratory protocols. Deparaffinization time and staining outcomes were compared. Results: Slides treated with EZ Prep cleaning solution for manual deparaffinization demonstrated staining quality comparable to xylene treatment across various special stains, including Gram's, GMS, PAS, Masson's trichrome, and Congo red. The difference in the excellent rate between the two methods is not statistically significant (χ ² = 0.33, P > 0.05). Moreover, the average deparaffinization time is significantly reduced from 33.0±2.7 minutes to 7.2±1.1 minutes, thereby speeding up the staining process. Conclusion: EZ Prep could replace xylene deparaffinized sections in special staining of ocular tissues with the advantages of shorter deparaffinized time and environmental protection, which is worthy of promotion and application.