目的：评估新一代基于人工智能(artificial intelligence,AI)的人工晶状体(intraocular lens,IOL)计算公式的准确性。方法：本研究为回顾性研究，纳入因白内障行晶状体超声乳化联合IOL植入术的262例患者262眼。在术前，通过IOLMaster700获取角膜曲率、角膜白到白、中央角膜厚度、前房深度、晶状体厚度以及眼轴长度。使用第三代公式(SRK/T、Holladay 1和Hoffer Q)、Barrett UniversalⅡ(BUⅡ)、新一代AI公式(Kane、Pearl-DGS、Hill-RBF 3.0、Hoffer QST和Jin-AI)对术后屈光状态进行计算，并与术后实际的屈光状态进行比较。在将预测误差(prediction error,PE)归零后，分析了各公式的标准差(standard deviation,SD)、绝对误差均值(mean absolute error,MAE)、绝对误差中位数(median absolute error,MedAE)以及PE在±0.25、±0.50、±1.00、±2.00 D范围内的百分比。结果：基于AI的IOL屈光力计算公式的SD、MAE和MedAE的范围分别为0.37 D(Kane和Jin-AI)至0.39 D(Hoffer QST)、0.28 D(Hill-RBF 3.0和Jin-AI)至0.31 D(Hoffer QST)以及0.21 D(Hill-RBF3.0和Jin-AI)至0.24 D(HofferQST)；均低于第三代公式(SD：0.43 D~0.45 D；MAE：0.34 D；MedAE：0.25 D~0.28 D)。在所有公式中，Jin-AI公式预测误差在±0.50 D的比例最高，为84.73%，Kane(84.35%)和BUⅡ(83.97%)公式次之。结论：在IOL屈光力预测上，与传统第三代公式相比，新一代基于AI的公式表现出更高的准确性，可以使更多的患者在术后获得预期的屈光状态。

Objective: To evaluate the accuracy of new generation artificial intelligence (AI)-based intraocular lens (IOL)power calculation formulas. Methods: This retrospective study included a total of 262 eyes from 262 patients with cataract who underwent uneventful phacoemulsification combined with IOL implantation. Keratometry, corneal white-to-white, central corneal thickness, anterior chamber depth, lens thickness, and axial length were measured by the IOL Master 700 before surgery. Predicted refractive errors were calculated by the third-generation formulas (SRK/T, Holladay 1, and Hoffer Q), Barrett UniversalⅡ (BUⅡ), and the newer-generation AI formulas (Kane, Pearl-DGS, Hill-RBF 3.0, Hoffer QST, and Jin-AI), and were compared with the actual postoperative refractive value. After adjusting the prediction error (PE) to zero, the standard deviation (SD), mean absolute error (MAE), median absolute error (MedAE), and the percentage of a PE within the range of ±0.25 diopter (D), ±0.50 D, ±1.00 D, and ±2.00 D were analyzed. Results: The SD, MAE, and MedAE of the AI-based formulas ranged from 0.37 D (Kane and Jin-AI) to 0.39 D (Hoffer QST), 0.28 D (Hill-RBF 3.0 and Jin-AI) to 0.31 D (Hoffer QST), and 0.21 D (Hill-RBF 3.0 and Jin-AI) to 0.24 D (Hoffer QST), respectively. These values were all lower than those of the third-generation formula (SD: 0.43 D to 0.45 D; MAE: 0.34 D; MedAE: 0.25 D to 0.28 D). Among all the formulas, the Jin-AI formula had the highest proportion of a PE within ±0.50 D (84.73%), followed by Kane (84.35%) and BUⅡ (83.97%) formulas. Conclusion: The new AI-based IOL formulas show higher accuracy compared with the traditional third-generation ones in predicting IOL power. thereby enabling more patients to achieve the expected refractive outcomes after surgery

目的: 建立人晶状体上皮细胞原代培养的简便方法并比较不同来源人晶状体上皮细胞的生物学特性。

方法: 取胎龄 20 周合法引产胚胎眼晶状体囊膜、中山眼科中心眼库眼晶状体囊膜和白内障患者术中撕取的前囊膜, 分别在培养皿中铺平, 加10 μL 10%DMEM 培养液润湿后加盖盖玻

片防止卷曲并促进粘贴, 添加培养液浸没盖玻片, 37℃培养。同时取相同来源的囊膜按照组织块法培养。观察细胞增殖情况并比较原代人晶状体上皮细胞与人晶状体上皮细胞系 SRA01/04 β晶体蛋白的表达差异。

结果: 在盖玻片辅助下, 胚胎眼晶状体囊膜第2天即可见明显的增殖细胞由囊膜缘长出, 眼库眼囊膜和白内障患者术中撕取的囊膜在3～4 d 的潜伏期后亦可见增殖细胞长出; 组织块法培养出现部分组织块漂浮, 且胚胎眼囊膜潜伏期延长至3～4 d, 眼库眼囊膜和白内障患者晶状体囊膜潜伏期延长至4～5 d。

结论: 盖玻片辅助的改良组织块培养法能尽快获得体外培养的原代晶状体上皮细胞, 且操作简便, 值得推广应用于晶状体病的研究。

Purpose: To set up an easy procedure of tissue culture for human lens epithelial cells in vitro and to observe the biological characteristics.

Methods: Capsules from embryo of 20 weeks, eye bank of Zhongshan Ophthalmic Centre and patients with cataract were spread on culture utensil. 10 μ L of 10% DMEM medium was added and a piece of coverslip was lay to prevent crimp. Then the capsules were cultured under 37℃after adding enough medium. Capsules from the same source were cultured by traditional tissue culture method. Expressions of β crystallin between primary tissue culture cells and SRA01/04 cell line were compared by western blotting.

Results: With coverslip assisted, the cells could be observed proliferated and migrated from the edge of embryo capsule 2 days later, and for capsules from eye bank and age-related cataract patients, the interval time was 3 to 4 days. By traditional tissue culture method, the interval time of embryo capsule was 3 to 4 days, and for capsules from eye bank and age-related cataract patients, the interval time was the same. And capsules floated sometimes.

Conclusions: By coverslip assisted primary tissue culture human lens epithelial cells could grow faster and easier, and the method is worthy to be spread in research of lens diseases.