目的：比较高碘酸－无色品红法（PAS）与改良的六胺银染色法（GMS）在真菌性角膜炎诊断应用中的优缺点。

方法：回顾性分析该科室自 2007 年 11 月至 2010 年 6 月诊断为真菌性角膜溃疡的 102 例标本，统计分析不同染色方法的阳性率，并对染色要点进行分析总结。

结果：GMS 对真菌性角膜炎的真菌显示率为 100％，PAS 则为 93.14％。

结论：GMS 对真菌性角膜炎的真菌显示效果优于 PAS，且较 PAS 染色省时、容易操作，不易褪色，不失为真菌染色的最佳选择。但是 PAS 染色显示真菌的分隔结构较为清晰，可排除色素细胞等的干扰，可以作为真菌性角膜炎鉴别诊断的补充染色。

Purpose: To compare the advantages and disadvantages of GMS and PAS staining in the diagnosis of fungal keratitis.

Methods: Retrospectively analyzed the sections of 102 cases of fungal keratitis from November 2007 to June 2010 in our department. Statistically analyzed the positive rate of different staining methods. And summarized technical cruxes of GMS and PAS staining.

Results: The positive rates of GMS staining and PAS staining for fungal keratitis were 100% and 93.14%, respectively.

Conclusions: GMS staining is better than PAS staining for fungal keratitis in displaying fungi. And it takes less time and is easier in operation and makes a more durable color than PAS staining. But PAS staining is better than GMS staining in displaying fungal septa and eliminating the interference from pigment-containing cells and other cells.

角膜是基因治疗的理想靶器官。角膜碱烧伤、角膜新生血管、角膜移植术后排斥反应因其病理机制复杂，所牵涉的致病因素众多而治疗困难，疗效不佳。本文就基因治疗在上述疾病中的应用加以综述，以了解基因治疗应用于角膜病变的新进展 。

Cornea is an ideal target organ for gene therapy. Corneal alkali burn, cornealneovascularization and corneal graft rejection tend to be with poor treatment elicacydue to its complex pathogenesis. This article aims to update the recent progress of genetherapy on corneal diseases.