目的：通过前房注射亚硒酸钠溶液建立新西兰兔硒性白内障模型，并评估验证其在药效试验中的应用。方法：采用前房注射10 mmol/L亚硒酸钠溶液(0.1 mL/只)对动物右眼进行造模，于造模第3天根据晶状体混浊度评分选取成模动物分为模型组、吡诺克辛滴眼液(pirenoxine sodium eye drops, PSED)0.05 mg/mL组，以模型组动物左眼作为空白组，每组8只眼；分组后对各组动物眼进行滴眼给药，50 µL/眼/次，3 次/天，连续给药17 d。于给药前、给药第7、17天进行裂隙灯检查拍照在体评估晶状体混浊度，末次给药后完整分离各组动物晶状体，体外评估晶状体透明度，然后将各组动物晶状体匀浆，用于过氧化物酶(peroxidase, POD)及谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)活力测定。结果：与空白组相比，模型组给药前、给药第7、17天晶状体混浊度评分均升高，给药结束后晶状体透明度评分升高，晶状体中GSH-PX和POD活力均降低；与模型组相比，PSED 0.05 mg/mL组给药17d晶状体混浊度和透明度评分均降低，晶状体中GSH-PX活力升高，POD无变化。结论：前房注射亚硒酸钠溶液可诱导新西兰兔发展出稳定的白内障模型症状，适用于药物药效作用的评价。

Objective: To establish a selenium-induced cataract model in New Zealand rabbits by anterior chamber injection of sodium selenite solution and evaluate and verify its application in pharmacodynamic trials. Methods: The right eyes of the animals were modeled by anterior chamber injection of 10 mmol/L sodium selenite solution (0.1 mL per animal). On the third day of modeling, the modeled animals were selected and divided into the model group and the pirenoxine sodium eye drops (PSED) 0.05 mg/mL group based on the lens turbidity score. The left eyes of the animals in the model group were taken as the blank group, with 8 eyes in each group. After grouping, eye drops were administered to the eyes of each group of animals. The dosage was 50 µL per eye each time, three times a day, for 17 consecutive days. Slit lamp examination and photography were conducted on the 7th and 17th days of administration to evaluate the turbidity of the lenses in vivo. After the last administration, the lenses of each group of animals were completely isolated, and the transparency of the lenses was evaluated in vitro. Then, the lenses of each group of animals were homogenized. It is used for the determination of peroxidase (POD) and glutathione peroxidase (GSH-Px) activities. Results: Compared with the blank group, the lens turbidity scores of the model group were significantly increased before administration, on the 7th and 17th days of administration, the lens transparency scores were significantly increased after the end of administration, and the activities of GSH-PX and POD in the lens were significantly decreased. Compared with the model group, the scores of lens turbidity and transparency in the PSED 0.05 mg/mL group and 17 days after administration were significantly decreased, the activity of GSH-PX in the lens was significantly increased, and there was no change in POD. Conclusions: Anterior chamber injection of sodium selenite solution can induce the development of stable cataract model symptoms in New Zealand rabbits and is suitable for the screening and evaluation of drug efficacy.