目的:探讨数字化裂隙灯显微镜作为一种定量分析的工具应用于一维尺寸测量的可靠性。
方法:3 位观察者采用单盲法,分别在 4 倍、10 倍、16 倍、25 倍、40 倍和 4 倍、10 倍、16 倍的物镜放大率下,对游标卡尺上模拟人眼的瞳孔和角膜直径的 4.00 mm 和 10.00 mm 间距,使用国产数字化裂隙灯显微镜进行图像采集和重复测量,对测量结果的正确度和精密度进行比较。
结果:3 位测量者(A、B、C)对 4.00 mm 尺寸的图像进行测量,所测结果均数位于 3.98~4.06 之间。对 10.00 mm 的图像进行测量,所测结果均数位于 10.00~10.04 之间。4.00 mm 测量结果显示,除测量者 A 4 倍、测量者 B 25 倍、测量者 C 16 倍和 25 倍外,测量值与真实值的差异具有统计学意义。10.00 mm 测量结果显示,除测量者 A 10 倍外,测量值与真实值的差异具有统计学意义。比较同一测量者对同一尺寸在不同放大倍率的测量结果,除测量者 A 对 10.00 mm 尺寸的测量无统计学差异外,其余测量结果之间在不同放大倍率下对相同尺寸测量的结果均具有统计学差异。不同测量者对相同物镜放大倍率下的同一尺寸比较,除在 4 倍放大率下对 4.00 mm 测量的结果无统计学差异外,其余测量结果之间的差异均具有统计学意义。所有测量结果的变异系数均低于 5%;随放大倍率的增加,变异系数有降低的趋势。
结论:数字化裂隙灯显微镜作为一种一维尺寸测量工具,具有良好的精密度,在利用其做定量分析前进行测量的可靠性分析有助于减少系统误差对测量结果分析的干扰。
Purpose: To study the reliability in one dimensional size measurement with digital slit lamp microscope as a tool for quantitative analysis.
Methods: Three single-blinded observers acquired and repeatedly measured the images of 4.00 mm and 10.00 mm size on the vernier caliper, which simulated the human eye pupil and cornea diameter, with home-made digital slit lamp microscope, respectively, in the objective magnification of 4 times, 10 times, 16 times, 25 times, 40 times and 4 times, 10 times, 16 times. The correctness and precision of measurement were compared.
Results: Measurements were conducted on the 4.00 mm image size by three investigators and all averages were located between 3.98 to 4.06, and for the 10.00 mm image size, between 10.00 to 10.04. Measurement results of 4.00 mm showed that, except for A4, B25, C16 and C25, the difference of the measured value and the true value was statistically significant. The measurement results of 10.00 mm showed that, except for A10, the difference of the measured value and the true value was statistically significant. Comparison of the same size, the same investigators at different magnification measurements, except for investigator A's measurements of 10.00 mm dimensions, had no significant difference; the others were all statistically significant. Compared the measurements of the same size with different magnification, except for measurements of 4.00 mm in 4-fold magnification, which had no significant difference among the investigators, the remaining different results were statistically significant. The coefficient of variation of all measurement results was less than 5%; as magnification increased, the coefficient of variation decreased.
Conclusion: The measurement of digital slit lamp microscope in one-dimensional size, with good reliability, should be used for analysis reliability before being used in quantitative analysis.
目的:探讨垂体腺瘤对眼视功能损害的临床表现。方法:对 126 例( 252 只眼)垂体腺瘤患者进行视力、视野、荧光眼底血管造影(Fundus fuorescein angiography, FFA)、图形视诱发电位(Pattem visual evoked potential, PVEP)及眼底检査。结果:视力下降 186 只眼,占73.8%。眼底原发性视神经萎缩 130 只眼,占51.6%。视野缺损 156 只眼,占69.6%。PVEP异常 160 只眼,占88.9%。26.2%的患者以眼部异常为首诊症状,其中16.7%曾被诊断为眼科疾病。结论:垂体腺瘤可引起视功能的损害,充分认识垂体腺瘤在眼部的临床特点,有助于早期诊断及时治疗。
Purpose : To investigate the clinical manifestation of damage of visual function causedby pituitary adenoma.Methods: Visual acuity, visual field, fundus fluorescein angiography (FFA), pattemvisual evoked potential (PVEP) and examination fundus were performed in 126 cases(252 eyes)of pituitary tumor.Results :There was 73.8% (186 eyes) of patients with decreased visual acuity, 51.6%(130 eyes) with primary optic atrophy, 69.6 %(156 eyes) with the defects of visualfield and 88.9%(160 eyes) with abnormal PVEP. Abnormal ophalmological manifestationwas the first diagnostic symptom in 26.2%, and 16.7% was misdiagnosed as eye diseases.Conclusions: Pituitary tumor could cause defection of visual function. lt is helpful toearly diagnosis and timely treatment by fully understanding clinical features in the eyewith pituitary tumor.