目的：比较高碘酸－无色品红法（PAS）与改良的六胺银染色法（GMS）在真菌性角膜炎诊断应用中的优缺点。

方法：回顾性分析该科室自 2007 年 11 月至 2010 年 6 月诊断为真菌性角膜溃疡的 102 例标本，统计分析不同染色方法的阳性率，并对染色要点进行分析总结。

结果：GMS 对真菌性角膜炎的真菌显示率为 100％，PAS 则为 93.14％。

结论：GMS 对真菌性角膜炎的真菌显示效果优于 PAS，且较 PAS 染色省时、容易操作，不易褪色，不失为真菌染色的最佳选择。但是 PAS 染色显示真菌的分隔结构较为清晰，可排除色素细胞等的干扰，可以作为真菌性角膜炎鉴别诊断的补充染色。

Purpose: To compare the advantages and disadvantages of GMS and PAS staining in the diagnosis of fungal keratitis.

Methods: Retrospectively analyzed the sections of 102 cases of fungal keratitis from November 2007 to June 2010 in our department. Statistically analyzed the positive rate of different staining methods. And summarized technical cruxes of GMS and PAS staining.

Results: The positive rates of GMS staining and PAS staining for fungal keratitis were 100% and 93.14%, respectively.

Conclusions: GMS staining is better than PAS staining for fungal keratitis in displaying fungi. And it takes less time and is easier in operation and makes a more durable color than PAS staining. But PAS staining is better than GMS staining in displaying fungal septa and eliminating the interference from pigment-containing cells and other cells.

       髓上皮瘤是源自神经系统的一种少见的恶性肿瘤，多发生在中枢神经系统和睫状体，而源自视神经的恶性髓上皮瘤则很少见，国内尚未有病例报道。此病早期类似胶质瘤，易造成误诊。本文报道了 1 例 3 岁 10 个月的男性患儿，经部分肿物切除活检发现肿瘤具有典型恶性髓上皮瘤的病理特点， 部分瘤细胞向软骨细胞分化，并逐渐形成透明软骨岛，NSE 及 S-100 表达阳性，病理诊断为源自视神经的畸胎性恶性髓上皮瘤。

       Medulloepithelioma is a clinically uncommon tumor originated from nervous system, often occurred in central nerve system and ciliary body, and malignant medulloepithelioma of the optic nerve is far rarer. So far, there has been no case report in China. It may be clinically misdiagnosed because it resembles glioma at the early stage of the disease. We reported a boy with a tumor in his right eye at age of 3.8 years, which was shown by biopsy of the partial tumor that there were some obviously heteromorphous neoplastic cells, karyokinesis, and moreover, some neoplastic cells differentiatied into cartilage cells, gradually formed into hyaline cartilage islands and the expressions of NSE and S-100 were positive. Teratoid malignant medulloepithelioma of optic nerve was made pathologically.