Purpose: To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells (RCECs) in vitro and explore its pharmacological mechanism.
Methods: The fresh rabbit cornea was cultured to get the primary RCECs, and RCECs of passage 2 were used for the research. The cells were divided into experimental groups, in which the cells were incubated with different concentrations (18.18, 27.27, 36.36, 45.45, 54.55 μg/ml) of diclofenac sodium, and a control group. The effect of diclofenac sodium on the proliferation of cells was measured by methyl thiazolyl tetrazolium (MTT) assay 24, 48, and 72 h after incubation. While the RCECs were divided into experimental groups, the cells in which were incubated with 9 and 12.5 μg / ml diclofenac sodium, and a control group. The cell cycle and apoptotic rate were observed by flow cytometer.
Results: MTT assay showed that diclofenac sodium had an obvious inhibitory effect on RCECs, and the inhibition rate was increasing along with the increase of the concentration of diclofenac sodium and the incubation time (P < 0.05). Flow cytometer showed that after incubation with diclofenac sodium, the cells in G0/G1 phase were obviously increased, and the apoptosis cusp and apoptotic rate were increased.
Conclusion: Diclofenac sodium has an obvious inhibitory effect on RCECs, which was dosage-dependent, and it may function by inducing cell apoptosis and ceasing cell cycles
Purpose: To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells (RCECs) in vitro and explore its pharmacological mechanism.
Methods: The fresh rabbit cornea was cultured to get the primary RCECs, and RCECs of passage 2 were used for the research. The cells were divided into experimental groups, in which the cells were incubated with different concentrations (18.18, 27.27, 36.36, 45.45, 54.55 μg/ml) of diclofenac sodium, and a control group. The effect of diclofenac sodium on the proliferation of cells was measured by methyl thiazolyl tetrazolium (MTT) assay 24, 48, and 72 h after incubation. While the RCECs were divided into experimental groups, the cells in which were incubated with 9 and 12.5 μg / ml diclofenac sodium, and a control group. The cell cycle and apoptotic rate were observed by flow cytometer.
Results: MTT assay showed that diclofenac sodium had an obvious inhibitory effect on RCECs, and the inhibition rate was increasing along with the increase of the concentration of diclofenac sodium and the incubation time (P < 0.05). Flow cytometer showed that after incubation with diclofenac sodium, the cells in G0/G1 phase were obviously increased, and the apoptosis cusp and apoptotic rate were increased.
Conclusion: Diclofenac sodium has an obvious inhibitory effect on RCECs, which was dosage-dependent, and it may function by inducing cell apoptosis and ceasing cell cycles
