目的：探讨环保试剂在眼科组织病理技术中的应用。方法：选取中山眼科中心2023年6月—2024年3月送检病理科手术标本190 例，一式两份分别采用环保试剂和传统试剂固定、脱水、透明、浸蜡、染色和封片，对两组试剂制片和染色的组织切片进行评价和判读、比较。结果：环保试剂组脱水、染色处理组织与传统试剂组相比，脱水效果良好，切片软硬适中，切面完整、无裂隙；苏木精-伊红(hematoxylineosin, HE)染色镜下可见细胞核结构清晰、核质对比鲜明、背景干净，两组优良片率分别为97.9%、98.4%，各级片差异无统计学意义(Z = –0.645，P=0.519)；免疫组织化学染色的组织抗原性完好，阳性信号强、定位准确，无背景染色。结论：应用环保试剂替代传统试剂在眼科组织标本中脱水、染色应用效果良好，可满足病理日常制片和染色需求，全流程无二甲苯，具有环保优点，成本可控，值得推广应用。

Objective: To investigate the utilization of environmentally-sustainable reagents in ophthalmic tissue pathology techniques. Methods: We prospectively collected 190 surgical specimens submitted to the pathology department of Zhongshan Ophthalmic Center between June 2023 and March 2024. Each specimen was divided into two sets, one treated with environmentally-sustainable reagents and the other with conventional reagents for fixation, dehydration, clearing, infiltration, staining, and mounting. The quality of histological sections from both groups was evaluated and compared. Results: Compared to the conventional reagent group, environmentally-sustainable reagent group showed good dehydration performance, moderately soft and hard tissue sections with intact cut surfaces and no cracks. H&E staining sections prepared with environmentally-sustainable reagents exhibited clear nuclear structures, distinct nuclear-cytoplasmic contrast, and clean backgrounds. The excellent section rates were 97.9% and 98.4% for the two, respectively. There was no significant difference in section quality between the two groups (Z = –0.645, P = 0.519). Tissue antigenicity was well-preserved during immunohistochemical procedures, with strong positive signals and accurate localization without background staining. Conclusions: The use of environmentally-sustainable reagents as a replacement for conventional reagents in the dehydration and staining of ophthalmic tissue yields satisfactory results, meeting the daily requirements for routine histological sectioning and staining. The entire process is xylene-free, environmentally-sustainable, cost-effective and worth being applied widely.

眼科病理学是系统组织病理学的重要分支，作为基础医学与临床医学之间的桥梁和纽带，对于深入认识和揭示眼部疾病的发病特点发挥着重要作用。眼科病理学在老一辈专家们的努力下奠定了坚实的基础。目前眼科病理学学科的发展随着眼科临床技术以及系统病理学的发展在不断的前进，细胞生物学及分子生物学的全方位结合和纵深应用带动并拓展了眼科病理学的研究领域，临床病理学及实验病理学的研究不断更新，新形势下眼科病理学的发展充满着机遇和挑战。

Ophthalmic pathology is an important branch of histopathology. As a bridge and link between basic medicine and clinical medicine, ophthalmic pathology plays an important role in understanding and revealing the characteristics of ocular diseases. The previous generations have laid a solid foundation for ophthalmic pathology with their hard work. Moreover, the advancement of ophthalmic clinical technology and systematic pathology in recent years have further facilitated the development of ophthalmic pathology. The comprehensive combination and in-depth application of cell biology and molecular biology have promoted and expanded the research field of ophthalmic pathology. In addition, new progress has been witnessed in the field of clinical pathology and experimental

pathology constantly. Under the new situation, the development of ophthalmic pathology is faced with both opportunities and challenges.