Purpose: To evaluate the clinical outcomes of tissue-engineered epithelium transplantation for severe ocular surface burns.
Methods: A retrospective observational case series. From october 2005 to May 2011, 19 eyes of l9 patients with grade IV to VI ocular surface burns (dua classification) were treated by autologous transplantation of corneal stem cells cultivated on a fibrin gel membrane, with a mean follow-up of 16. 2 months (range 12-36 months) postoperative corneal surface stability, visual acuity (VA), corneal opacity, and neovascularization were evaluated.
Results: No corneal perforation occurred and the entire corneal surface was free from epithelial defects in all eyes. At the final follow-up visit, visual acuity in 17 eyes was improved after surgery, with 6 eyes achieving a VA of 20/100 or better. The corneal vascularization was obviously reduced in 17 (89.5%) eyes. The corneal opacity was also improved in 12 (63. 2%) eyes. All donor eyes remained healthy.
Conclusion: Tissue-engineered epithelium transplantation can promote rapid reepithelialization of the ocular surface, inhibit corneal neovascularization, and improve vision for patients with severe ocular surface burns.

Purpose: To evaluate the clinical outcomes of tissue-engineered epithelium transplantation for severe ocular surface burns.

Methods: A retrospective observational case series. From october 2005 to May 2011, 19 eyes of l9 patients with grade IV to VI ocular surface burns (dua classification) were treated by autologous transplantation of corneal stem cells cultivated on a fibrin gel membrane, with a mean follow-up of 16. 2 months (range 12-36 months) postoperative corneal surface stability, visual acuity (VA), corneal opacity, and neovascularization were evaluated.
Results: No corneal perforation occurred and the entire corneal surface was free from epithelial defects in all eyes. At the final follow-up visit, visual acuity in 17 eyes was improved after surgery, with 6 eyes achieving a VA of 20/100 or better. The corneal vascularization was obviously reduced in 17 (89.5%) eyes. The corneal opacity was also improved in 12 (63. 2%) eyes. All donor eyes remained healthy.
Conclusion: Tissue-engineered epithelium transplantation can promote rapid reepithelialization of the ocular surface, inhibit corneal neovascularization, and improve vision for patients with severe ocular surface burns.

A 55-year-old male complained of right eye blurry vision for 3 days. His best-corrected visual acuity (BCVA) was 0.2 for the right eye and 1.0 for the left eye. Anterior segment and vitreous body examinations of both eyes were normal. Yellowish-white focal lesions in the macula of the right eye were observed and subtly changes of lesions were found along the superotemporal and inferotemporal arcades in the macula two days later. Fluorescein fundus angiography (FFA) revealed slight ffuorescent leakage from the lesions in the macula of the right eye, and segmental venous leakage and optic disc hyperffuorescence were observed in both eyes. Indocyanine green angiography (ICGA) demonstrated that the lesions in the macula of the right eye had hypofluorescence at a late stage and spectral domain optical coherence tomography (SD-OCT) imaging of the macula showed focal impairment of the inner segment and outer segment (IS/OS). The blood investigation indicated a positive treponema pallidum hemagglutination assay (TPPA) and a rapid plasma reagin test (RPR) of 1:32. After antisyphilitica treatment for 6 weeks, the yellowish-white lesions had vanished and the BCVA was 1.2 followed by restoration of the IS/OS for the right eye, with an RPR of 1:4. In conclusion, ophthalmologists should alert unilateral focal lesions in the macula may be the ffrst sign of syphilis. Prompt treatment is highly effective in resolving vision.

A 55-year-old male complained of right eye blurry vision for 3 days. His best-corrected visual acuity (BCVA) was 0.2 for the right eye and 1.0 for the left eye. Anterior segment and vitreous body examinations of both eyes were normal. Yellowish-white focal lesions in the macula of the right eye were observed and subtly changes of lesions were found along the superotemporal and inferotemporal arcades in the macula two days later. Fluorescein fundus angiography (FFA) revealed slight ffuorescent leakage from the lesions in the macula of the right eye, and segmental venous leakage and optic disc hyperffuorescence were observed in both eyes. Indocyanine green angiography (ICGA) demonstrated that the lesions in the macula of the right eye had hypofluorescence at a late stage and spectral domain optical coherence tomography (SD-OCT) imaging of the macula showed focal impairment of the inner segment and outer segment (IS/OS). The blood investigation indicated a positive treponema pallidum hemagglutination assay (TPPA) and a rapid plasma reagin test (RPR) of 1:32. After antisyphilitica treatment for 6 weeks, the yellowish-white lesions had vanished and the BCVA was 1.2 followed by restoration of the IS/OS for the right eye, with an RPR of 1:4. In conclusion, ophthalmologists should alert unilateral focal lesions in the macula may be the ffrst sign of syphilis. Prompt treatment is highly effective in resolving vision.