目的：建立高效液相色谱法(high-performance liquid chromatography, HPLC)测定曲伏前列素滴眼液中曲伏前列素含量。方法：采用Dikma C18色谱柱(50 mm×4.6 mm, 3 μm)；以磷酸溶液(取磷酸1.0 mL，用水稀释至1 000 mL，用1 mol/L氢氧化钠溶液调节pH至2.8)-乙腈(67:33)为流动相；流速为每分钟3.0 mL；柱温为25℃；检测波长为220 nm；进样体积30 μL。结果：曲伏前列素在20.28~70.98 μg /mL(r = 0.999 5)范围内线性关系良好，平均回收率为100.3%，相对标准偏差(relative standard deviatio, RSD)为2.0% (n = 9)，该方法重现性好。对照品溶液和供试品溶液在室温放置48 h基本稳定。结论：该方法可用于曲伏前列素滴眼液中的曲伏前列素含量测定。

Objective: To establish a high-performance liquid chromatography (HPLC) method for the determination of content of Travoprost in Travoprost Eye Drops. Methods: The analytic column was Dikma C18 (50 mm×4.6 mm, 3 μm) . Using phosphoric acid solution (take 1.0 mL of phosphoric acid, dilute with water and make up to 1 000 mL, adjust the pH to 2.8 with 1 mol/L sodium hydroxide solution)-acetonitrile (67:33) as mobile phase. The flow rate is 3.0 mL/min. The column temperature is 25 ℃; The detection wavelength is 220 nm. The injection volume is 30 μL. Results: The linear range of travoprost showed were well shown within 20.28-70.98 μg/mL(r=0.998). The average recovery rate of travoprost was 100.3% with relative standard deviation (RSD) 2.0% (n=9). The method had high reproducibility. The reference solution and the test solution remain stable at room temperature for 48 hours. Conclusion: The method can be used for the determination of content of travoprost in Travoprost Eye Drops.

目的：建立高效液相色谱法(high-performance liquid chromatography，HPLC)测定盐酸丁卡因滴眼液中盐酸丁卡因和羟苯乙酯的含量。方法：采用Agilent Eclipse PLUS C18色谱柱(250 mm ×4.6 mm，5 μm)；以1%三乙胺溶液(三乙胺10 mL，加水990 mL，用冰醋酸调节pH值至5.0±0.5)-乙腈(65 : 35)为流动相，流速为1.0 mL/min；柱温为30 ℃；检测波长为256 nm；进样体积20 μL。结果：盐酸丁卡因在0.05~0.36 mg/mL范围内线性关系良好( r =1.000)，平均回收率为99.2%，相对标准偏差(relative standard deviation，RSD)为0.3%(n=9)，羟苯乙酯在3.02~24.14 μg/mL范围内线性关系良好(r=1.000)，平均回收率为98.2%，RSD为0.4%(n=9)，该方法重现性好。对照品溶液和供试品溶液在室温放置24 h基本稳定；结论：本方法简便、快速、准确。适用于检测盐酸丁卡因滴眼液中盐酸丁卡因和羟苯乙酯的含量。

Objective: To establish a high-performance liquid chromatography (HPLC) method for the determination of tetracaine hydrochloride and ethyl hydrobenzoate in tetracaine hydrochloride eye drops. Methods: The analytic column was Agilent Eclipse Plus C18 (4.6 mm ×250 mm, 5 μm) and the mobile phase was 1% triethylamine solution (10 mL triethylamine and 990 mL water, pH adjusted to 5.0±0.5 with glacial acetic acid) - acetonitrile (65:35) at a flow rate of 1.0 mL/min. The detection wavelength was 256 nm and the column temperature was 30 ℃. The injection volume was 20 μL. Results: The linear range of tetracaine hydrochloride was well shown within 0.05–0.36 mg/mL (r=1.000). The average recovery rate of tetracaine hydrochloride was 99.2% with relative standard deviation (RSD) 0.3% (n=9). The linear range of ethyl hydrobenzoate was well shown within 3.02–24.14 μg/mL (r=1.000). The average recovery rate of tetracaine hydrochloride was 98.2% with RSD 0.4%(n=9). The method had high reproducibility. The reference solution and testing solution were stable for 24 h in room. Conclusion: The method is simple, quick and accurate, which is suitable for the determination of tetracaine hydrochloride and ethyl hydrobenzoate in tetracaine hydrochloride eye drops.