角膜是基因治疗的理想靶器官。角膜碱烧伤、角膜新生血管、角膜移植术后排斥反应因其病理机制复杂，所牵涉的致病因素众多而治疗困难，疗效不佳。本文就基因治疗在上述疾病中的应用加以综述，以了解基因治疗应用于角膜病变的新进展 。
Cornea is an ideal target organ for gene therapy. Corneal alkali burn, cornealneovascularization and corneal graft rejection tend to be with poor treatment elicacydue to its complex pathogenesis. This article aims to update the recent progress of genetherapy on corneal diseases.
目的：观察 NIDEK EC5000 准分子激光治疗系统准分子激光原位角膜磨镶术(Laser in sitkeratomileusis, LASIK) 角膜切削深度的可预测性。
方法：采用 NIDEK EC5000 准分子激光系统对 79 例近视和(或)近视散光患者进行标准 LASIK 手术，术中使用超声角膜测厚仪分别测量制瓣后和激光切削后的剩余角膜床厚度，计算实际角膜切削深度，比较实际角膜切削深度同理论预测角膜切削深度的差异。
结果：LASIK 术中实际切削深度(92.32±29.86) μm，预测切削深度(74.16±25.95) μm，两者差值(18.16 ± 14.71) μm 有统计学意义(P < 0.001)。实际切削深度与预测切削深度具有较好的相关性，相关系数为0.87 (P < 0.001)，其直线回归方程为Y = 18.06 + 1.001X。按术前角膜 K 值、术前等效球镜绝对值及术前中央角膜厚度值分组的实际切削深度与预测切削深度的差值均有统计学意义。实际切削深度与术前等效球镜有关，与术前中央角膜厚度和 K 值无关。实际切削深度与预测切削深度差值同 K 值、等效球镜，术前中央角膜厚度均无关。
结论：NIDEK EC5000 准分子激光系统 LASIK 术中实际角膜切削深度比预测角膜切削深度高 (18.16±14.71) μm，在手术设计时要考虑实际切削与机器标示值存在偏差，应尽可能多的预留剩余角膜基质床厚度，以提高手术安全性。
Purpose: To assess the predictability of corneal ablation depth in LASIK using NIDEK EC5000 excimer laser.
Method: Standard LASlK surgery was performed in 79 myopic patients with or without astigmatism with the NDEK EC5000 excimer laser system. Ultrasonic cornealpachymetry was performed immediately after flap creation and after laser ablation during LASIK procedure, by which the actual corneal ablation depth was calculated.The values of actual and predicted ablation depth were compared.
Results: The actual ablation depth was (92.32+29.86) μm, the predicted ablationdepth was (74.16+25.95) μm. The differences between them (18.16+14.71) μm were statistically significance (P < 0.001 ). Linear regression suggested that the actual ablation depth correlated closely with the predicted ablation depth (r = 0.87 , P < 0.001 ). The regression model was Y = 18.06+1.001X. The differences remained statistically significant and were independent of the levels of preoperative corneal keratometry, absolute preoperative spherical equivalent and the preoperative central cornea thickness.
Conclusion: The actual ablation depth was about (18.16+14.71) μm thicker than thepredicted ablation depth in the NlDEK EC5000 excimer laser system. We may have totake into account this deviation in order to ensure sufficient thickness of residualstromal bed.
Purpose: To investigate the effect of intravitreal injection of basic fibroblast growth factor (bFGF) on activation and proliferation of endogenous retinal progenitor cells in the Royal College of Surgeons (RCS) rat.
Methods: Twenty-four rats were studied after the 30th postnatal day (≥30). Eighteen RCS-p+/LAV rats were divided into 3 groups: bFGF-treated, vehicle-treated, and untreated groups randomly, and 6 RCS-ray+p+/Lav rats were used as normal controls. 6 μl of bFGF (5 μg /10 μl) or vehicle was injected into the vitreous on day 31, 33, and 35 after birth (P31, P33, P35) in the bFGF group and vehicle group respectively, and no injections were administered in the untreated and control groups. All the rats were euthanized, and their eyes were enucleated, hemisected, and fixed at 50 days after birth for immunohistochemistry and measurement of outer nuclear layer thickness.
Results: Nestin and Chx10 were positive in all retinal layers. Intravitreal injection of bFGF in retina-dystrophic RCS (RCS-p+/Lav) rats induced intense labeling for the retinal progenitor cell markers Chx10 and Nestin, which were highly colocalized. Fluorescence intensity for both labels was somewhat less in the control rats, and much less in the vehicle-injected rats as well as in the untreated RCS rats. The outer nuclear layer (ONL) was significantly thicker in the bFGF group than that in the vehicle-treated or untreated group (P < 0.01), but thinner than that of the control group (P < 0.01). No significant difference was observed in the ONL thickness between the vehicle group and untreated group (P > 0.05).
Conclusion: bFGF may contribute to the activation of retinal progenitor cells in RCS rats, thus counteracting degeneration by promoting the proliferation of the progenitor cells.
Purpose: To investigate the effect of intravitreal injection of basic fibroblast growth factor (bFGF) on activation and proliferation of endogenous retinal progenitor cells in the Royal College of Surgeons (RCS) rat.
Methods: Twenty-four rats were studied after the 30th postnatal day (≥30). Eighteen RCS-p+/LAV rats were divided into 3 groups: bFGF-treated, vehicle-treated, and untreated groups randomly, and 6 RCS-ray+p+/Lav rats were used as normal controls. 6 μl of bFGF (5 μg /10 μl) or vehicle was injected into the vitreous on day 31, 33, and 35 after birth (P31, P33, P35) in the bFGF group and vehicle group respectively, and no injections were administered in the untreated and control groups. All the rats were euthanized, and their eyes were enucleated, hemisected, and fixed at 50 days after birth for immunohistochemistry and measurement of outer nuclear layer thickness.
Results: Nestin and Chx10 were positive in all retinal layers. Intravitreal injection of bFGF in retina-dystrophic RCS (RCS-p+/Lav) rats induced intense labeling for the retinal progenitor cell markers Chx10 and Nestin, which were highly colocalized. Fluorescence intensity for both labels was somewhat less in the control rats, and much less in the vehicle-injected rats as well as in the untreated RCS rats. The outer nuclear layer (ONL) was significantly thicker in the bFGF group than that in the vehicle-treated or untreated group (P < 0.01), but thinner than that of the control group (P < 0.01). No significant difference was observed in the ONL thickness between the vehicle group and untreated group (P > 0.05).
Conclusion: bFGF may contribute to the activation of retinal progenitor cells in RCS rats, thus counteracting degeneration by promoting the proliferation of the progenitor cells.
Purpose: To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells (RCECs) in vitro and explore its pharmacological mechanism.
Methods: The fresh rabbit cornea was cultured to get the primary RCECs, and RCECs of passage 2 were used for the research. The cells were divided into experimental groups, in which the cells were incubated with different concentrations (18.18, 27.27, 36.36, 45.45, 54.55 μg/ml) of diclofenac sodium, and a control group. The effect of diclofenac sodium on the proliferation of cells was measured by methyl thiazolyl tetrazolium (MTT) assay 24, 48, and 72 h after incubation. While the RCECs were divided into experimental groups, the cells in which were incubated with 9 and 12.5 μg / ml diclofenac sodium, and a control group. The cell cycle and apoptotic rate were observed by flow cytometer.
Results: MTT assay showed that diclofenac sodium had an obvious inhibitory effect on RCECs, and the inhibition rate was increasing along with the increase of the concentration of diclofenac sodium and the incubation time (P < 0.05). Flow cytometer showed that after incubation with diclofenac sodium, the cells in G0/G1 phase were obviously increased, and the apoptosis cusp and apoptotic rate were increased.
Conclusion: Diclofenac sodium has an obvious inhibitory effect on RCECs, which was dosage-dependent, and it may function by inducing cell apoptosis and ceasing cell cycles
Purpose: To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells (RCECs) in vitro and explore its pharmacological mechanism.
Methods: The fresh rabbit cornea was cultured to get the primary RCECs, and RCECs of passage 2 were used for the research. The cells were divided into experimental groups, in which the cells were incubated with different concentrations (18.18, 27.27, 36.36, 45.45, 54.55 μg/ml) of diclofenac sodium, and a control group. The effect of diclofenac sodium on the proliferation of cells was measured by methyl thiazolyl tetrazolium (MTT) assay 24, 48, and 72 h after incubation. While the RCECs were divided into experimental groups, the cells in which were incubated with 9 and 12.5 μg / ml diclofenac sodium, and a control group. The cell cycle and apoptotic rate were observed by flow cytometer.
Results: MTT assay showed that diclofenac sodium had an obvious inhibitory effect on RCECs, and the inhibition rate was increasing along with the increase of the concentration of diclofenac sodium and the incubation time (P < 0.05). Flow cytometer showed that after incubation with diclofenac sodium, the cells in G0/G1 phase were obviously increased, and the apoptosis cusp and apoptotic rate were increased.
Conclusion: Diclofenac sodium has an obvious inhibitory effect on RCECs, which was dosage-dependent, and it may function by inducing cell apoptosis and ceasing cell cycles
目的：评估低视力青少年使用电子助视器对其阅读速度的影响。
方法：在泉州市盲校筛查 10 名使用光学助视器有阅读能力的低视力青少年学生进行屈光矫正和常规外眼、内眼检查，分别测量其使用各种光学助视器及电子助视器阅读5号字卡的阅读速度。
结果：10 名低视力青少年学生在使用光学助视器后阅读速度为 (18.50±6.54) 字/分，使用电子助视器后阅读速度为 (34.36±5.06) 字/分，两组比较差异有统计学意义 (P < 0.05)。低视力青少年的近视力与阅读速度无关 (P > 0.05)。
结论：使用电子助视器较光学助视器可以明显提高低视力青少年的阅读速度，不同病因所致的低视力青少年其近视力与阅读速度无关。
Purpose: To evaluate the reading speed of adolescents with different causes of low vision using electronic visual aids.
Methods: The screening of 10 young students from Quanzhou Blind School who could read Chinese N5 print by different optical aids. After refractive correction and ophthalmic examination, the reading speeds with Chinese N5 print were measured using various optical and electronic visual aids.
Results: The reading speed of (34.36 ± 5.06) words/min by electronic visual aids performed faster than the reading speed of (18.50 ± 6.54) words/min by optical visual aids in 10 young students with low vision (P < 0.05). The reading speed of young people with low vision due to different causes had no direct linear correlation with their near visual acuity (P > 0.05).
Conclusion: The electronic visual aids could more significantly improve the reading speed of young people with low vision than the optical visual aids did. The reading speed of young people with low vision due to different causes was not related to their near visual acuity.
目的：探讨复合小梁切除术后并发浅前房的原因、治疗和预后情况。方法：对在该院行复合小梁切除术的 267 例（302 眼）青光眼患者进行回顾性研究 ，观察术后浅前房的发生原因、治疗、预后情 况。结果：302 眼中有 43 眼出现浅前房（1424％）。 术后滤过过强、滤过口渗漏，脉络膜脱离及睫状环阻滞是浅前房的主要原因，浅前房发生后给予保守或手术治疗，43 眼前房均恢复正常。结论：复合小梁切除术后浅前房是常见的一种并发症。术中控制滤过率，合理应用丝裂霉素可减少浅前房的发生；术后密切观测前房、眼压和滤过情况，及早对浅前房进行干预可明显改善预后。
Purpose: To investigate the clinical cause, therapy and prognosis of shallow anterior chamber after complex trabeculectomy surgery of glaucoma.
Methods: A retrospective study of 267 patients (302 eyes) diagnosed with glaucoma at our hospital was reviewed.Result: Forty-three among 302 eyes presented shallow anterior chamber (14.24%). The main causes of shallow anterior chamber after surgery included excessive filtering function, conjunctival exudation, choroidal detachment and ciliary circle block. These patients recovered after receiving conservative or surgical treatment.Conclusions: The shallow anterior chamber is a frequent complication occurring following complex trabeculectomy surgery. The occurrence of this complication may be decreased by controlling filterableness and moderately applying mitomycin C intraoperatively; the prognosis may be significantly enhanced by detecting main measurements including anterior chamber, intraocular tension, filtration condition, and by interfering in shallow anterior chamber early after complex trabeculectomy surgery.
目的：观察羊膜移植手术对于减少碱烧伤后角膜新生血管的疗效。
方法：回顾性病例对照研究。2006－2010 年期间该院收治的 Ⅲ 度角膜碱烧伤的患者 19 例 23 眼，其中行羊膜移植术（治疗组）11 例 13 眼，未行羊膜移植术（对照组）8 例 10 眼。该两组的年龄和手术外的处理基本匹配。伤后 3 d 治疗组行羊膜移植术。分别在伤后 14、60 d 测量各组角膜新生血管面积。应用 SPSS 12.0 统计学软件将此两组的面积进行配对 t 检验，以 P < 0.05 作为差异有统计学意义。
结果：在烧伤后 14 d 治疗组新生血管面积（62.133 ± 8.571）mm2，明显低于对照组（89.561 ± 9.741）mm2，治疗组较对照组减低 30.6%，两组差异具有统计学意义（P < 0.05）。烧伤后 60 d 治疗组新生血管面积（112.019 ± 17.362）mm2，明显低于对照组（129.481 ± 13.534）mm2，治疗组较对照组减低 13.5%，两组差异具有统计学意义（P < 0.05）。
结论：羊膜移植术能明显抑制碱烧伤所致角膜新生血管的生长。
Purpose: To study the curative effect of amnion membrane transplantation on decreasing corneal neovascularization (CNV) induced by alkali burn.
Methods: It was a non-randomized retrospective case-control study. Among 19 cases (23 eyes) of third-degree alkali burns from 2006 to 2010, 11 cases (13 eyes) were performed with amnion membrane transplantation operation, and others were not. Amnion membrane transplantation was performed at the 3rd day after burn in the treatment group. Ages and treatments beyond surgery of double groups were matched. Areas of CNV in double groups were measured at the 14th day and 60th day after burn.
Results: Area of CNV in the treatment group was (62.133 ± 8.571) mm2 at the 14th day after burn, and was 30.6% lower than that in the control group. Area of CNV in the treatment group was (112.019 ± 17.362) mm2 at the 60th day after burn, and was 13.5% lower than that in the control group. There was statistical significance (P < 0.05).
Conclusion: Amnion membrane transplantation operation can inhibit the growth of corneal neovascularization induced by alkali burn.
目的：观察和分析儿童眼科门诊就诊的屈光不正 3～7 岁患儿，有早产史和足月产史的患儿的屈光不正的特点和差异。
方法：屈光不正 179 例（358 眼），分为 2 组：早产史者 51 人，足月产者 128 人。1％阿托品眼膏散瞳进行视网膜带状光剪影验光。
结果：足月儿的屈光不正患儿中，以远视多见，占 157／256 眼（61.3％），对比有早产儿屈光不正的远视发病 25／102（24.5％），差异有统计学意义（P < 0.05）。有早产儿屈光不正中，以散光发病为主，占 81／102 眼（79.4％），尤以高度散光、混合散光多见，相对与足月儿，其散光发病，高度散光发病和混合散光发病眼数的差异均有显著性（P < 0.05）。
结论：散光，尤其是高度散光、复杂的混合散光是有早产儿童视力低下的重要原因。临床上散光与弱视的形成关系密切相关，因此不能忽略早产儿童视力发育，最早可提前到 2 岁即可进行屈光筛查。
Purpose: To observe the abnormal refractive state and clinical characteristics in preterm and full-term children of the Department of Pediatric Ophthalmology.
Methods: The ocular refraction status of 358 eyes in 51 preterm and 128 full-term children were checked by retinoscopy in dilated pupil after being used atropine eye drops.
Results: There were 157 eyes with hyperopia accounting for 61.3% in preterm children, and 25 eyes with hyperopia accounting for 24.5% in full-term children. The main type of refractive errors in preterm children is astigmatism, especially in high astigmatism and mixed astigmatism. The morbidity of astigmatism, hyper astigmatism, and mixed astigmatism in preterm children is higher than that in full-term children.
Conclusion: Astigmatism, especially high astigmatism and complex mixed astigmatism, are important reasons for low vision in preterm children. Clinically, there is a close relationship between astigmatism and amblyopia. Therefore, the visual development of preterm children should not be ignored, and refractive screening could be brought forward to two years old.
目的：探讨正常成年 SD 大鼠的明视视网膜电图（Electroretinogram，ERG）特征。
方法：选取正常 9～12 周 SD 大鼠 60 只，使用罗兰视觉电生理仪记录大鼠右眼的明视闪光 ERG。使用 SPSS 统计分析 a 波、b 波和明视负波反应（Photopic negative response，PhNR）的隐含期和振幅。比较雄性和雌性 SD 大鼠明视 ERG 特征。
结果：每只 SD 大鼠均能记录到稳定的 a 波、b 波和 PhNR，其中 a 波的隐含期和 PhNR 的隐含期及振幅均符合正态分布，而其余指标均不符合正态分布。PhNR 的隐含期为 124.6±8.5 ms，其变异系数最小（0.07）。PhNR 的振幅为（11.3±4.2）μV，变异系数为 0.37。雄性和雌性 SD 大鼠明视 ERG 的各反应波之间无显著差异。
结论：在正常成年 SD 大鼠，明视闪光 ERG 是一项客观评价大鼠明视状态下视网膜功能的手段，PhNR 可以作为一项稳定的评价内层视网膜功能的指标。
Purpose: To study the characteristics of photopic flash electroretinogram (ERG) in normal adult SD rats.
Methods: Sixty normal adult SD rats aged 9 to 12 weeks old were enrolled in this study. Photopic flash ERG was recorded from these 60 SD rats. The results were analyzed with SPSS.
Results: Stable a, b and PhNR waves could be recorded in each rat. The implicit time of a wave, implicit time and amplitude of PhNR fit normal distribution. The implicit time of PhNR was (124.6 ± 8.5) ms with the smallest coefficient of variation of 0.07. The amplitude of PhNR was (11.3 ± 4.2) μV and the coefficient of variation was 0.37. There was no difference between the results of female and male rats.
Conclusion: Photopic flash ERG is an objective method in evaluating the retinal function in rats and PhNR can be used as a sensitive index of inner retinal function.
