Objective To explore the use of indocyanine green lens anterior capsular membrane staining in cataract?phacoemulsification and to assess its safety and efficacy. Methods Sixty-six cases (80 eyes) of white cataract patients who underwent cataract phacoemulsification as inpatients in Jiangyin People's Hospital from December 2022 to December 2023 were selected and divided into 40 eyes in the control group and 40 eyes in the stained group according to whether or not the anterior capsule membrane of indocyanine green lens was stained or not in the intraoperative period, and intraoperative recordings were made on the success of capsule tearing, time of capsule tearing, and the occurrence of complications in the two groups, and postoperative follow-ups were made to track the best-corrected visual acuity, intraocular pressure(IOP), corneal endothelial cell counts, and complications in the two groups at the first day, week, month, and month, respectively; and the above indexes were analyzed statistically. Results The intraoperative time for continuous circular capsulorhexis(CCC) in the stained group was significantly less than that in the control group and the success rate of capsular tearing (85%) was significantly higher than that in the control group, both of which were statistically significant (t=6.508, 2=11.168, P<0.05); The incidence of intraoperative radial tearing of the tearing capsule incision, failure to CCC and change the truncated capsule approach, and intraocular lens ciliary sulcus implantation was significantly lower in the stained group than in the control group (2=5.000, 5.230, 4.501, P<0.05); The incidence of postoperative corneal oedema, anterior chamber exudation and elevated IOP in the staining group were not significantly higher in the staining group compared with the control group; there was no significant difference between the best-corrected visual acuity and IOP of the patients of the two groups in the different periods of time. In the staining group, the number of corneal endothelial cells lost in the first week after surgery was less than that in the control group (t=2.708, P<0.05), while the difference in the number of corneal endothelial cells lost in the first month and the third month after surgery was not statistically significant between the two groups. Conclusion Indocyanine green lens anterior capsular membrane staining can improve the success rate of CCC and reduce intraoperative complications in cataract echo-emulsification in the absence of fundus red light reflex without obvious toxic side effects, making it a safe and efficient adjunct.
Abstract Objective To investigate the correlation between the expression level, survival prognosis and immune invasion of TBX2 in uveal melanoma. Methods The expression and clinical features of TBX2 were analyzed by TIMER2.0 database, and the survival and prognosis of TBX2 were analyzed by UCSC database. Then cBioPortal database was used to analyze the mutation survival changes of human TBX2, and BloodSpot and TIMER2.0 databases were used to explore the correlation between TBX2 and cancer immune infiltration. The relationship between its expression and molecular mechanism was studied by single cell State Mapping and Gene Set Variation analysis (GSVA). Results TBX2 mRNA expression levels were significantly changed in 15 tumor types, and TBX2 was a typical prognostic marker for ACC, UVM and KIRP. The mutation had no significant correlation with survival status, but was associated with T cells. In addition, the TBX2 pathway is enriched to ABC transporters, DNA repair, and damage in UVM. Conclusion TBX2 plays a key role in the survival and immune invasion of Uveal melanoma.
Objective: To investigate a technique for fixation and preparation of frozen sections of whole mouse eyeballs. Methods: Three male C57BL/6 mice, aged 2 and 24 months, were taken from the general class and the eyeballs were removed after anaesthesia, killed and fixed in picric acid fixative for 2 hours to prepare frozen sections. Subsequently, hematoxylin-eosin staining was performed and the preparation of frozen sections of whole mouse eyeballs was observed under a light microscope. Results: The mouse eyeballs obtained by this method were basically undistorted, and the whole layer of mouse eyeball sections was observed to be complete and clear under the microscope. The structure of the cornea was clear, the outline of the lens was basically complete, and the ciliary processes and ciliary zonules were visible. The structure of the four cell layers in the retina was clear and without fissures, the nuclei and nuclear membranes were clear and blue, and the cytoplasm of the cells was pink with bright colours. There were many pigment cells in the choroid and the structure was continuous and complete. Conclusions: In conclusion, the frozen sections of mouse eyeballs prepared by the above method were satisfactory and simple, which laid a certain foundation for good scientific research conditions.
The training of professional master students in ophthalmology (professional master) is faced with many restriction. The 3D Surgery Joint Teaching Laboratory teaching method (Wet-Lab) realizes the anatomy of the eye, the rehearsal of the operation and the simulation of the clinical process, etc. Its repeatability and 3D authenticity can not only deepen the learning impression and improve the operation level of the master of ophthalmology, but also greatly increase the learning interest, providing a new teaching mode for training qualified ophthalmologists.
A 30-year-old male patient had poor vision from an early age and planned to undergo transparent lens replacement surgery locally. After eye examination and evaluation, he was diagnosed as bilateral pupil residual membrane and bilateral ametropia. The patient underwent one-stage resection of residual pupil membrane and two-stage TICL implantation, and the visual acuity recovered well after operation. In this paper, the treatment process of this patient was reviewed in order to provide reference for the diagnosis and treatment of clinical ametropia with pupillary residual membrane.
