Purpose: To conduct a review to systematically evaluate the use of anterior segment opticalcoherence tomography (AS-OCT) in measuring anterior scleral thickness across diverse ocularconditions and its clinical implications. Methods: Literature search was conducted across electronicdatabases, including PubMed, Scopus, and Embase, to identify relevant studies. The risk of biaswas assessed, and the main characteristics of each  studies were analyzed. We calculated theoverall mean anterior scleral thickness using the data which have measurement at the same locations. Results: A total of 32 studies were included that utilized AS-OCT to measure anterior scleralthickness in both healthy subjects and individuals with ocular disorders such as myopia, keratoconus, scleritis, and others , The review found that anterior scleral thickness is signiicantly influenced by age, diurnal variation, and specific ocular conditions. For example, myopic eyes mayexhibit thinner sclera, particularly along certain meridians, while conditions like scleritis showecincreased scleral thickness due to inflammation, However, some studies have inconsistent resultsAdditionally, AS-OCT proved effective in detecting subtle variations in anterior scleral thickness. which could be linked to the progression of ocular diseases. Conclusions: Anterior scleral thicknessvaries considerably depending on age, time of day, and ocular health, making it a valuable parameterin the assessment of eye conditions. AS-OCT's ability to measure these variations non-invasivelybroadens its application in both clinical practice and research, offering new insights into thebiomechanical properties of the sclera and their implications for ocular diseases.

Purpose: To conduct a review to systematically evaluate the use of anterior segment opticalcoherence tomography (AS-OCT) in measuring anterior scleral thickness across diverse ocularconditions and its clinical implications. Methods: Literature search was conducted across electronicdatabases, including PubMed, Scopus, and Embase, to identify relevant studies. The risk of biaswas assessed, and the main characteristics of each  studies were analyzed. We calculated theoverall mean anterior scleral thickness using the data which have measurement at the same locations. Results: A total of 32 studies were included that utilized AS-OCT to measure anterior scleralthickness in both healthy subjects and individuals with ocular disorders such as myopia, keratoconus, scleritis, and others , The review found that anterior scleral thickness is signiicantly influenced by age, diurnal variation, and specific ocular conditions. For example, myopic eyes mayexhibit thinner sclera, particularly along certain meridians, while conditions like scleritis showecincreased scleral thickness due to inflammation, However, some studies have inconsistent resultsAdditionally, AS-OCT proved effective in detecting subtle variations in anterior scleral thickness. which could be linked to the progression of ocular diseases. Conclusions: Anterior scleral thicknessvaries considerably depending on age, time of day, and ocular health, making it a valuable parameterin the assessment of eye conditions. AS-OCT's ability to measure these variations non-invasivelybroadens its application in both clinical practice and research, offering new insights into thebiomechanical properties of the sclera and their implications for ocular diseases.

随着人工智能(artificial intelligence，AI)技术的快速发展，基于深度学习(deep learning，DL)和机器学习的AI技术在医学领域上的应用受到了广泛的关注。AI在眼科的应用也逐渐向更全面更深入的层次发展，通过角膜断层扫描、光学相干断层扫描、裂隙灯图像等技术，AI在对角膜病变、结膜病变、白内障、青光眼等眼部疾病的诊断和治疗方面都表现出了良好的性能。然而AI在眼科的应用方面也存在一些诸如结果可解释性的欠缺、数据集标准化的缺乏、数据集质量的不齐、模型适用性的不足和伦理问题等挑战。在5G和远程医疗飞速发展的时代，眼科AI同时也有许多新的机遇。本文综述了AI在前段眼科疾病中的应用、临床实施的潜在挑战和前景，为AI在眼科领域的进一步发展提供参考信息。

With the rapid development of artificial intelligence (AI) technology, the application of AI technology based on deep learning (DL) and machine learning (ML) in the medical field has received widespread attention. The application of AI in ophthalmology is gradually being shifted to a more comprehensive and in-depth level. Trained on corneal tomography, optical coherence tomography (OCT), slit-lamp images, and other techniques. AI can achieve robust performance in the diagnosis and treatment of corneal lesions, conjunctival lesions, cataract, glaucoma and other ophthalmic diseases. However, there are also some challenges in the application of AI in ophthalmology, including the lack of interpretability of results, lack of standardization of data sets, uneven quality of data sets, insufficient applicability of models and ethical issues. In the era of 5G and telemedicine, there are also many new opportunities for ophthalmic AI. In this review, we provided a summary of the state-of-the-art AI application in anterior segment ophthalmic diseases, potential challenges in clinical implementation and its development prospects, and provides reference information for the further development of artificial intelligence in the field of ophthalmology.

视神经属于中枢神经的一部分，损伤后难以再生。视神经损伤通常伴随视网膜神经节细胞(retinal ganglion cells，RGCs)的持续性凋亡及视神经变性坏死，引起视力损害甚至完全失明。目前针对视神经再生的基础研究主要集中于保护和维持视神经损伤后RGCs的存活、促进RGCs轴突再生及重建视神经功能。本文以RGCs保护、轴突再生及视神经功能重建等为关键词，查询国内外最新视神经再生研究类文献，并分析整理，从抗氧化应激、提供外源性细胞因子、炎症刺激、抗胶质瘢痕、基因调控等方面阐述近年的视神经再生研究进展，以期对后续的基础研究开展及临床转化有所帮助。

Optic nerves are a part of the central nervous system, which is difficult to regenerate after injury. Optic nerve injury is usually accompanied by continuous apoptosis of retinal ganglion cells (RGCs) and degeneration or necrosis of optic nerves, resulting in visual impairment or even complete blindness. At present, the basic research on optic nerve regeneration mainly focuses on protecting and maintaining the survival of RGCs after optic nerve injury, promoting RGCs axon regeneration, and reconstructing optic nerve function. In this paper, RGCs protection,axon regeneration, and optic nerve function reconstruction are used as key words to collect the latest domestic and foreign literatures on optic nerve regeneration. The research progress of optic nerve regeneration in recent years was reviewed from the aspects of antioxidant stress, provision of exogenous cytokines, inflammatory stimulation, anti-glial scar, gene regulation and so on, in order to help the follow-up basic research and clinical translation.

目的：分析喉罩在肥胖患者眼科日间手术中的应用特点。方法：选择 2021 年 1 月— 2024 年 3 月在中山大学中山眼科中心行日间眼科手术 且体质量指数（body mass index，BMI）≥25 kg/m²的男性患者进行分析，将患者分为2组：超重组（BMI 25~<30 kg/m2）及肥胖组（BMI≥30 kg/m²）。比较两组患者入室时（T₁）、诱导后（T₂）、喉罩置入后（T₃）、手术开始时（T₄）、手术开始10 min（T₅）、手术结束时（T₆）、入麻醉恢复室（post-anesthesia care unit，PACU）（T₇）、喉罩拔除时（T₈）的血流动力学和血氧饱和度变化情况；并分析喉罩置入后（T₃）、手术开始时（T₄）、手术开始10 min（T₅）、手术结束时（T₆）和喉罩拔除时（T₈）患者的呼吸参数变化情况，记录两组患者的喉罩通气成功率及复苏室的停留时间。结果：纳入 118 例患者，其中超重组 57 例、肥胖组 61 例。两组均未出现喉罩通气失败病例，肥胖组在T₃、T₄、T₅、T₆以及T₈等时点气道峰压均高于超重组（P < 0.05）；肥胖组的呼吸道不良事件发生率高于超重组（P < 0.05）。两组患者围术期生命体征平稳（P > 0.05），复苏室停留时间在组间比较差异无统计学意义（P > 0.05）且均顺利日间手术离院。结论：喉罩通气下全身麻醉能安全应用在肥胖患者眼科日间手术中，高BMI与高气道峰压相关。

Objective: General anesthesia using laryngeal mask airway (LMA) ventilation can facilitate ambulatory ophthalmic surgery, however, there remains debates about the use of LMA for obese patients. This study retrospectively analyzed the application of LMA in obese patients for ambulatory ophthalmic surgery. Methods: In this retrospective study, we analyzed all patients with a BMI≥25 kg/m2 who underwent ambulatory ophthalmic surgery under general anesthesia at the Zhongshan Ophthalmic Center of Sun Yat-sen University from January 2021 to March 2024.  The patients were divided into two groups: overweight group (BMI 25~<30 kg/m2) and obese group (BMI ≥30 kg/m2) . We compared the changes in hemodynamics and oxygen saturation at the several time points, baseline after entering operating room (T₁), anesthesia induction (T₂), LMA insertion (T₃), start of surgery (T₄), 10 minutes after surgery (T₅), surgery completion (T₆), transfer to PACU (T₇), and LMA removal (T₈);and analyzed the changes in respiratory parameters of the patient at T₃, T₄, T₅, T₆, and T₈ time points between both groups. We also observed the differences in success rate of LMA insertion and ventilation and PACU stay time as well as time to discharge home between two groups. Results: 118 cases were found suitable for the final analysis and divided into overweight group (n=57) and obese group (n=61). All patients in the two groups uneventfully completed the ophthalmic ambulatory surgery and discharged home under the laryngeal mask airway ventilation, none of patients in both group displayed LMA ventilation failure. The hemodynamics and oxygen saturation in both groups were stable during perioperative period(P > 0.05). The peak airway pressures in obese group were dramatically increased than in overweight group at T₃, T₄, T₅, T₆ and T8 time points (P < 0.05), and the incidences of adverse respiratory events in obese group were significant higher than in overweight group （P < 0.05）. The PACU stay time was comparable in both groups (P > 0.05). Conclusion: LMA can be safely used for obese patients undergoing ophthalmic surgery, high BMI is related to high peak airway pressures.

Abstract: Retinal degeneration, a leading cause of irreversible blindness, often results from photoreceptor deterioration due to genetic mutations or damage to retinal pigment epithelial (RPE) cells. Emerging research highlights the gut-eye axis, a connection between gut microbiota and eye diseases. Our recent findings demonstrate that bacterial translocation from the gut to the retina contributes to Crb1-associated retinal degeneration. This review explores the relationship between the gut microbiome and retinal degeneration, focusing on inherited retinal degeneration (IRD) and age-related macular degeneration (AMD).

Abstract: Retinal degeneration, a leading cause of irreversible blindness, often results from photoreceptor deterioration due to genetic mutations or damage to retinal pigment epithelial (RPE) cells. Emerging research highlights the gut-eye axis, a connection between gut microbiota and eye diseases. Our recent findings demonstrate that bacterial translocation from the gut to the retina contributes to Crb1-associated retinal degeneration. This review explores the relationship between the gut microbiome and retinal degeneration, focusing on inherited retinal degeneration (IRD) and age-related macular degeneration (AMD).

人工智能(artificial intelligence，AI)在白内障手术中的应用越来越广泛，二者结合对于白内障手术的术前诊断和分级管理、术中人工晶状体选择、位置预测及术后管理(视力预测、并发症预测及随访)、手术培训和教学方面均起到巨大的促进作用。诚然，AI在与白内障手术相关的管理、分析和研究中还面临着许多问题，但其广泛的应用前景不可忽视。现对AI在白内障手术治疗和教学中的应用做以总结，并对其未来的发展做出展望。

Artificial intelligence (AI) has been widely used in cataract surgery. The combination of the two can play a great role in improving preoperative diagnosis, grading management of cataract surgery, intraoperative intraocular lens selection and location prediction, postoperative management (vision prediction, complication prediction and follow-up), surgical training and teaching. It is true that AI still faces many problems in the management, analysis and research related to cataract surgery, but its broad application prospects cannot be ignored. This review summarizes the application of AI in cataract surgery and teaching, and the future prospects of AI.

目的：利用生物信息学方法分析与葡萄膜恶性黑色素瘤转移相关的非编码RNA，以及它们作为竞争性内源RNA的作用机制。方法：从癌症基因组图谱(The Cancer Genome Atlas，TCGA)数据库下载80例葡萄膜恶性黑色素瘤患者的RNA测序数据和临床资料，采用edgeR算法分析转移与非转移患者组织中差异表达(differentially expressed，DE)的长链非编码RNA(lncRNA)、微小RNA(miR)和mRNA，并构建lncRNA-miR-mRNA的竞争性内源RNA(competing endogenous RNA，ceRNA)调控网络，基因富集分析和通路分析研究网络中mRNA的生物学功能。Kaplan-Meier生存曲线分析ceRNA网络中核心RNA与生存率的关系。结果：从发生远处转移的葡萄膜恶性黑色素瘤样本中，共鉴定出346个上调的mRNA，118个下调的miR和45个上调的lncRNA。其中67个mRNA，7个miR和30个lncRNA相互组合形成616个ceRNA单元，并形成了一个具有181条边线ceRNA网络。基因富集分析表明：网络中的mRNA富集在肿瘤生成和转移相关的几个基因本体(Gene Ontology)和信号通路。拓扑分析确定了6个核心lncRNA(LINC00861、LINC02421、BHLHE40-AS1、LINC01252、LINC00513和LINC02389)和3个核心mRNA(UNC5D、BCL11B和MTDH)。 所有核心lncRNA、核心mRNA的表达水平和5个miR(miR-221、miR-222、miR-506、miR-507、miR-876)的表达水平均与总体生存率显着相关(均P<0.05)。结论：本研究揭示了几种lncRNA及其相关的ceRNA网络在葡萄膜恶性黑色素瘤转移中的作用，为进一步研究葡萄膜恶性黑色素瘤的发生和/或转移提供了新的方向。

Objective: To elucidate the expression of long non-coding RNAs (lncRNAs) and their roles as competing endogenous RNAs (ceRNAs) in uveal melanoma (UM) metastasis. Methods: RNA sequencing data and clinical information of 80 patients with UM were obtained from The Cancer Genome Atlas (TCGA) database. Differentially expressed (DE) mRNAs, microRNAs (miR), and lncRNAs between metastatic and non-metastatic individuals with UM were screened using the edgeR algorithm. Gene enrichment analysis was conducted for the DE mRNAs. LncRNA-miR-mRNA regulatory triples and a ceRNA network were constructed. Betweenness centrality was used to screen hub genes and lncRNAs for subnetwork analysis. Kaplan-Meier survival analysis was conducted to explore correlations between the expression of hub RNAs and overall survival in the TCGA UM cohort. Results: A total of 346 upregulated mRNAs, 118 downregulated miRs, and 45 upregulated lncRNAs were identified in samples with systemic metastasis. Among them, 67 mRNAs, 7 miRs, and 30 lncRNAs mapped to 616 ceRNA triples, thus forming an interconnected ceRNA network with 181 edges. Gene enrichment analysis revealed that mRNAs in the network were enriched in multiple gene ontology terms and pathways associated with carcinogenesis and metastasis. Topological analysis identified 6 hub lncRNAs (LINC00861, LINC02421, BHLHE40-AS1, LINC01252, LINC00513, and LINC02389) and 3 hub mRNAs (UNC5D, BCL11B, and MTDH). The expression levels of all hub genes and 5 DEmiRs (miR-221, miR-222, miR-506, miR-507, miR-876) were significantly associated with the overall survival probability. Conclusion: This bioinformatic study revealed the functions of several lncRNAs and their associated ceRNA network in UM metastasis. It provides a novel in silicon evidence for future experimental study on the pathogenesis of systemic metastasis in uveal melanoma, especially from the perspective of non-coding RNA.

目的：建立高效液相色谱法(high-performance liquid chromatography, HPLC)测定曲伏前列素滴眼液中曲伏前列素含量。方法：采用Dikma C18色谱柱(50 mm×4.6 mm, 3 μm)；以磷酸溶液(取磷酸1.0 mL，用水稀释至1 000 mL，用1 mol/L氢氧化钠溶液调节pH至2.8)-乙腈(67:33)为流动相；流速为每分钟3.0 mL；柱温为25℃；检测波长为220 nm；进样体积30 μL。结果：曲伏前列素在20.28~70.98 μg /mL(r = 0.999 5)范围内线性关系良好，平均回收率为100.3%，相对标准偏差(relative standard deviatio, RSD)为2.0% (n = 9)，该方法重现性好。对照品溶液和供试品溶液在室温放置48 h基本稳定。结论：该方法可用于曲伏前列素滴眼液中的曲伏前列素含量测定。

Objective: To establish a high-performance liquid chromatography (HPLC) method for the determination of content of Travoprost in Travoprost Eye Drops. Methods: The analytic column was Dikma C18 (50 mm×4.6 mm, 3 μm) . Using phosphoric acid solution (take 1.0 mL of phosphoric acid, dilute with water and make up to 1 000 mL, adjust the pH to 2.8 with 1 mol/L sodium hydroxide solution)-acetonitrile (67:33) as mobile phase. The flow rate is 3.0 mL/min. The column temperature is 25 ℃; The detection wavelength is 220 nm. The injection volume is 30 μL. Results: The linear range of travoprost showed were well shown within 20.28-70.98 μg/mL(r=0.998). The average recovery rate of travoprost was 100.3% with relative standard deviation (RSD) 2.0% (n=9). The method had high reproducibility. The reference solution and the test solution remain stable at room temperature for 48 hours. Conclusion: The method can be used for the determination of content of travoprost in Travoprost Eye Drops.

Amblyopia is a neurodevelopmental vision disorder resulting from abnormal visual input during the critical period of visual development, such as strabismus, uncorrected anisometropia, high refractive errors, and form deprivation. It is frequently associated with reduced visual acuity and deficits in binocular vision. Traditional occlusion therapy for amblyopia has typically been restricted to infants and young children during the critical period of visual development, as it is believed to be ineffective for older children and adults due to the decreased plasticity of the mature brain. Our research group has concentrated on pivotal scientific issues in amblyopia, including quantitative methods for detecting binocular vision, especially interocular visual suppression, the mechanisms underlying binocular vision impairment in amblyopia,treatment methods and their evaluations for amblyopia, and visual plasticity and its neural mechanismsin amblyopia. This paper summarizes the visual mechanisms and treatment modalities of amblyopia based on our research and both domestic and foreign sources, while also looking forward to the future development of this field in light of existing problems.

Amblyopia is a neurodevelopmental vision disorder resulting from abnormal visual input during the critical period of visual development, such as strabismus, uncorrected anisometropia, high refractive errors, and form deprivation. It is frequently associated with reduced visual acuity and deficits in binocular vision. Traditional occlusion therapy for amblyopia has typically been restricted to infants and young children during the critical period of visual development, as it is believed to be ineffective for older children and adults due to the decreased plasticity of the mature brain. Our research group has concentrated on pivotal scientific issues in amblyopia, including quantitative methods for detecting binocular vision, especially interocular visual suppression, the mechanisms underlying binocular vision impairment in amblyopia,treatment methods and their evaluations for amblyopia, and visual plasticity and its neural mechanismsin amblyopia. This paper summarizes the visual mechanisms and treatment modalities of amblyopia based on our research and both domestic and foreign sources, while also looking forward to the future development of this field in light of existing problems.

目的：探究囊袋张力环(CTR)植入对五种新一代人工晶状体(IOL)计算公式[Barrett Universal Ⅱ (BUⅡ), Emmetropia Verifying Optical (EVO), Kane, Pearl-DGS和Hill-RBF 2.0]在高度近视患者中预测准确性的影响。方法：前瞻性病例对照研究。观察2020年12月—2021年9月于陕西省眼科医院就诊的眼轴长度(axial length,AL)≥ 27.00 mm行白内障联合IOL(AR40E, 美国强生)植入术的患者。术眼随机分为植入CTR组(A组)和未植入CTR组(B组)。术前根据IOLMaster700测量眼部参数，使用BU Ⅱ公式计算所需IOL度数。记录术后1周、1个月及3个月实际等效球镜度(spherical equivalent,SE)，计算并比较五种公式预测误差(prediction error,PE)和绝对屈光预测误差(absolute Error,AE)。将A组和B组分别分为A1组(27.00 mm ≤ AL ≤ 30.00 mm)和A2组(AL>30.00 mm)；B1组(27.00 mm ≤ AL ≤ 30.00 mm)和B2组(AL >30.00 mm)，分析不同AL范围内CTR植入对公式预测准确性的影响。结果：共纳入患者63例(89眼)，年龄(55.93±10.17)岁，术前AL为(30.30±2.18)mm。A组、A1组及A2组术后不同时间SE值比较差异均无统计学意义(P>0.05)，B组、B1组及B2组术后1周与1个月，术后1周与3月SE值分别比较差异有统计学意义(P<0.05)，术后1个月与3个月比较，差异无统计学意义(P>0.05)。A组、B组、A1组、A2组、B1组和B2组各组中五种公式的AE值比较差异均无统计学意义(均P>0.05)。植入CTR后五种公式的预测误差变化比较差异无统计学意义(P>0.05)。结论：对于AL ≥27.00 mm的白内障患者，植入CTR组术后1周屈光度趋于稳定，未植入组术后1个月屈光度趋于稳定。CTR植入对五种公式预测准确性和选择无影响，五种计算公式均可正常选择。

Objective: To investigate the predictive accuracy and effect of capsular tension ring (CTR) implantation with five new generation intraocular lens (IOL) calculation formulas [Barrett Universal Ⅱ (BU Ⅱ), Emmetropia Verifying Optical(EVO), Kane, Pearl-DGS and Hill-RBF 2.0] in high myopia patients. Methods: This is a prospective case-control study. The patients were enrolled with an axial length (AL)≥27.00 mm, and underwent cataract surgery with AR40E IOL implantation at the Shaanxi Eye Hospital from December 2020 to September 2021. The patients were randomly assigned to the CTR implantation group (group A) and the non-CTR implantation group (group B). With the ocular parameters measured by the IOLMaster700, the IOL power was calculated with the BUⅡformula before surgery. The postoperative actual equivalent spherical diopter (SE) were recorded,and the predicted error (PE) and absolute error (AE) using the five formulas were recorded and compared at 1 week, 1 month, and 3 months, repsectively. Group A was divided to A1 (27.00 mm ≤ AL ≤ 30.00 mm) and A2 (AL>30.00 mm), and group B was divided to B1 (27.00 mm ≤ AL ≤ 30.00 mm) and B2 (AL>30.00 mm). The effects of CTR implantation and the accuracy of the formulas were analyzed with different AL ranges. Results: A total of 63 patients (89 eyes) were included, aged (55.93±10.17) years old, with preoperative AL (30.30± 2.18)mm. There was no statistically significant difference in SE between groups A, A1, and A2 (P>0.05) at different postoperative times. While there was a statistically significant difference in SE between groups B, B1, and B2 (P < 0.05) at 1 week and 1 month after surgery, and between 1 week and 3 months after surgery. There was no statistically significant difference between 1 month and 3 months after suergery (P>0.05). There was no significant difference in the AE using the five formulas among groups A, B, A1, A2, B1, and B2 (P>0.05). There was no statistically significant difference in prediction error changes among the five formulas after CTR implantation (P>0.05). Conclusion: For cataract patients with AL ≥ 27.00 mm, the refractionvalue in the CTR implantation group tended to stabilizeafter one week of surgery. While in the non-CTR implantation group, the refractionvalue tended to stabilize after one month. CTR implantation had no effect on the accuracy and selection of the five formula, and the five IOL calculation formulas can be normally selected.